##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	TSS1-2-C-GII-rdrp_S71_L001_R2_001_fastq_gz.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104136
Sequences flagged as poor quality	0
Sequence length	79-301
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.23886072059614	34.0	34.0	34.0	31.0	34.0
2	32.90135976031343	34.0	34.0	34.0	32.0	34.0
3	33.416080894215256	34.0	34.0	34.0	33.0	34.0
4	33.02673427056926	34.0	34.0	34.0	31.0	34.0
5	31.872906583698242	34.0	34.0	34.0	23.0	34.0
6	36.774640854267496	38.0	38.0	38.0	35.0	38.0
7	36.21318276100484	38.0	36.0	38.0	31.0	38.0
8	34.94936429284781	38.0	37.0	38.0	24.0	38.0
9	35.21142544365061	38.0	38.0	38.0	26.0	38.0
10-14	35.420920719059694	37.8	37.2	38.0	27.6	38.0
15-19	35.27882768687101	38.0	38.0	38.0	26.8	38.0
20-24	35.04201236844127	38.0	36.2	38.0	25.8	38.0
25-29	34.79709418452792	38.0	36.2	38.0	25.2	38.0
30-34	35.26304640086042	38.0	36.8	38.0	26.6	38.0
35-39	34.340510486287165	37.2	34.8	38.0	25.0	38.0
40-44	33.310330721364366	37.0	31.6	38.0	23.2	38.0
45-49	33.94950449412307	37.0	33.0	38.0	24.6	38.0
50-54	34.51794768379811	37.4	34.0	38.0	27.0	38.0
55-59	34.51250672197895	37.4	34.8	38.0	25.8	38.0
60-64	34.508617577014675	38.0	35.6	38.0	23.6	38.0
65-69	33.71242221710072	37.6	33.4	38.0	22.8	38.0
70-74	34.11035184758393	37.2	34.4	38.0	24.6	38.0
75-79	33.8527368057156	37.2	33.4	38.0	25.0	38.0
80-84	33.708789552023816	37.0	33.2	38.0	23.2	38.0
85-89	34.06858597013492	38.0	34.4	38.0	22.8	38.0
90-94	33.44260047054304	37.6	34.0	38.0	18.6	38.0
95-99	32.30800595380995	36.6	29.6	37.8	19.8	38.0
100-104	33.08504729437749	37.0	31.4	38.0	20.0	38.0
105-109	32.38364622845345	36.6	29.8	38.0	19.4	38.0
110-114	31.76313626263599	36.2	28.0	37.8	15.2	38.0
115-119	31.3052452656244	35.0	27.8	37.6	16.2	38.0
120-124	31.646522169190728	36.0	28.4	37.6	14.8	38.0
125-129	31.10564769377035	35.2	26.6	37.8	15.8	38.0
130-134	29.397539637571906	32.4	24.6	36.8	15.2	38.0
135-139	31.588314718959772	36.4	28.2	37.8	13.2	38.0
140-144	31.52832105712996	36.4	27.6	37.6	15.6	38.0
145-149	31.606338170189474	35.6	28.4	37.6	16.0	38.0
150-154	30.978500158454256	35.2	27.0	37.6	16.2	37.8
155-159	30.434475804515465	35.6	25.4	37.4	12.2	38.0
160-164	27.10433972592216	29.2	19.2	35.8	11.4	37.4
165-169	28.313622264263284	31.4	23.4	35.8	12.8	37.4
170-174	26.379412470830015	29.6	17.0	36.0	9.8	37.6
175-179	27.503936387819188	31.2	19.2	36.8	9.8	37.4
180-184	24.693776108939698	25.2	15.2	34.8	9.2	36.6
185-189	26.074951743476966	29.0	17.0	35.4	9.8	37.0
190-194	23.750541145288146	25.2	13.6	33.2	9.6	36.4
195-199	27.906759754539955	31.0	20.6	36.4	10.0	37.2
200-204	24.589120256549396	26.6	16.6	33.6	8.2	36.4
205-209	25.585335638144628	28.0	17.0	35.4	9.0	37.2
210-214	21.399546720445596	21.8	12.4	29.8	8.0	34.6
215-219	20.00724481850331	19.6	11.8	27.6	8.6	33.6
220-224	21.20103322530238	21.4	10.6	30.0	8.6	34.8
225-229	20.617444116172713	20.4	11.4	28.8	8.0	34.0
230-234	20.83450545700902	20.6	12.4	28.4	8.6	32.8
235-239	15.737701139876867	14.0	9.6	20.8	8.2	26.2
240-244	14.204629043464834	12.4	8.0	19.0	7.2	24.8
245-249	13.428626572994673	11.4	7.8	16.4	7.8	23.0
250-254	11.918848143287269	10.4	7.0	14.6	7.0	20.8
255-259	15.81952939081653	14.2	9.8	20.8	7.4	26.2
260-264	11.288870360076585	9.2	7.0	14.0	7.0	19.2
265-269	12.372598974739052	10.6	7.8	15.6	7.6	20.6
270-274	11.137372614415417	9.0	8.0	13.8	7.4	17.0
275-279	10.759440429868445	8.8	7.2	12.8	7.0	17.8
280-284	9.6522759557779	8.0	7.0	12.0	7.0	13.2
285-289	10.65257859304552	8.8	7.0	12.4	7.0	17.0
290-294	11.22843098523013	8.6	7.8	13.8	7.4	19.0
295-299	10.322686989512516	8.2	7.4	12.6	7.0	16.0
300-301	9.523567575671361	8.0	7.0	12.0	7.0	13.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.12962227934716708
1101	2	0.22623858878787217
1101	3	-0.05072836263003211
1101	4	0.010975168963170745
1101	5	0.14186345202169903
1101	6	0.08622879129660532
1101	7	0.23718776082436932
1101	8	-0.12205367227453934
1101	9	-0.52370588283096
1101	10-14	-0.062054828436217235
1101	15-19	0.1733171953259074
1101	20-24	-0.14288499981974923
1101	25-29	0.13982420916995864
1101	30-34	-0.24304901159373316
1101	35-39	0.1938005291390752
1101	40-44	0.09725751983481246
1101	45-49	0.3292083660376335
1101	50-54	0.3648754890478614
1101	55-59	0.6248161578705478
1101	60-64	0.3125952951373705
1101	65-69	0.4312634217992297
1101	70-74	0.4570908779686036
1101	75-79	0.2713158729686569
1101	80-84	0.15134442545558358
1101	85-89	0.13634853143161507
1101	90-94	0.05153849261819943
1101	95-99	-0.2746353569085187
1101	100-104	0.18628429866343765
1101	105-109	0.29726816876227957
1101	110-114	0.427192223694437
1101	115-119	0.3694974464447469
1101	120-124	-0.0671914686815569
1101	125-129	0.14215902317340934
1101	130-134	-0.8827195336295297
1101	135-139	-0.21386195642563166
1101	140-144	-0.18447885687154297
1101	145-149	-0.098603717339941
1101	150-154	-0.4577915017035359
1101	155-159	-0.38978137422913406
1101	160-164	-1.1682236439433886
1101	165-169	-0.3772695842491025
1101	170-174	-0.6958915715173681
1101	175-179	-0.7226330372954486
1101	180-184	-0.456584960850396
1101	185-189	-1.0123427206641047
1101	190-194	-0.6825247621243378
1101	195-199	-0.6137164331178901
1101	200-204	-1.0348999763592666
1101	205-209	-0.8941961032399455
1101	210-214	-1.0235479802598455
1101	215-219	-0.9549587396444075
1101	220-224	-1.7112674957329759
1101	225-229	-1.1983268267027718
1101	230-234	-1.5491622317416756
1101	235-239	-0.21957229273061607
1101	240-244	-0.5742525405350989
1101	245-249	-0.5607153847978914
1101	250-254	-0.25073895250307743
1101	255-259	0.8639445890109609
1101	260-264	-0.3925642782546621
1101	265-269	-0.3494363599256527
1101	270-274	0.0012565837429896476
1101	275-279	-0.6358046051737674
1101	280-284	-0.23861340680714882
1101	285-289	0.050210939594101944
1101	290-294	0.3004295408986799
1101	295-299	-0.47074965094468446
1101	300-301	-0.06819134236172886
1102	1	0.4800557095341631
1102	2	0.6141555920310608
1102	3	0.4944653246009594
1102	4	0.3284100338703979
1102	5	0.5494566184130747
1102	6	0.49333969878728823
1102	7	0.7863239145685057
1102	8	-0.23812133319830764
1102	9	0.06352872406775134
1102	10-14	0.05428772006095528
1102	15-19	0.494395526227855
1102	20-24	0.3268713388706814
1102	25-29	0.0785315141866576
1102	30-34	-0.0011117775896565263
1102	35-39	-0.10736774304785257
1102	40-44	-0.39010850261433916
1102	45-49	-0.42188334754879975
1102	50-54	-0.4433060332023686
1102	55-59	0.30080328155789715
1102	60-64	-0.07284530865076988
1102	65-69	-0.34970664197952317
1102	70-74	0.016506380180970837
1102	75-79	-0.30427218147836754
1102	80-84	0.018279549576959653
1102	85-89	0.4176115674925285
1102	90-94	-0.24454918204995124
1102	95-99	-0.19809363548536751
1102	100-104	0.07299686030214758
1102	105-109	-0.5284748113565989
1102	110-114	-0.5629359366084152
1102	115-119	-0.3836076980520744
1102	120-124	-0.5640821044193558
1102	125-129	-0.0653015220202775
1102	130-134	-0.735141076134628
1102	135-139	-0.6865938325333225
1102	140-144	-0.5335354378970649
1102	145-149	-0.017680673804019165
1102	150-154	-0.3666952066575391
1102	155-159	-0.4123848489045123
1102	160-164	-1.0871486888537518
1102	165-169	-0.9885761441757985
1102	170-174	-1.7501360526670737
1102	175-179	-1.4575497873527148
1102	180-184	-0.9964328081672313
1102	185-189	-0.957748601811403
1102	190-194	-2.123671091531282
1102	195-199	-1.1132546702204742
1102	200-204	-1.4209578715402955
1102	205-209	-0.5795872754907698
1102	210-214	-1.418267245297283
1102	215-219	-1.653713306033378
1102	220-224	-1.3716656004554935
1102	225-229	-1.8810800427668113
1102	230-234	-1.4728158521931007
1102	235-239	-0.18914412396859248
1102	240-244	-0.8018083545926338
1102	245-249	-1.0448055821044981
1102	250-254	-0.5229281162988819
1102	255-259	-0.6123070328404072
1102	260-264	-0.386971687319134
1102	265-269	-0.23535645298636787
1102	270-274	0.08428195173547337
1102	275-279	-0.19491605432592962
1102	280-284	0.11145594132045034
1102	285-289	-0.10420273317221707
1102	290-294	1.9427289960439182
1102	295-299	0.8293018117777766
1102	300-301	-0.0552642172407829
1103	1	0.10037557754859705
1103	2	0.05308148558076198
1103	3	0.20161002855683563
1103	4	0.33363035866324964
1103	5	0.08157269676719636
1103	6	0.3354248738036034
1103	7	0.3071744335229667
1103	8	-0.5352489233535564
1103	9	0.32650258550752653
1103	10-14	0.12582833147917682
1103	15-19	0.3508735306848294
1103	20-24	-0.10097613899835522
1103	25-29	0.0077641018179264165
1103	30-34	0.15874589468721467
1103	35-39	-0.1302485251116039
1103	40-44	0.1643170769771416
1103	45-49	-0.21174557529778326
1103	50-54	-0.47203013164980945
1103	55-59	0.06942715961899637
1103	60-64	0.06925833449570007
1103	65-69	-0.9122532740081795
1103	70-74	-0.3250407566432614
1103	75-79	-0.0876287025751381
1103	80-84	-0.11002963154615486
1103	85-89	0.23941381075473345
1103	90-94	-0.3531664115841835
1103	95-99	-0.47780701010523785
1103	100-104	-0.4332933848315079
1103	105-109	-0.2821124173944476
1103	110-114	-0.20708461736832007
1103	115-119	-0.6623878164943555
1103	120-124	0.19067836393243098
1103	125-129	2.6410410985278077E-5
1103	130-134	-0.5298394915690565
1103	135-139	-1.0301946254317045
1103	140-144	-1.0528100097442525
1103	145-149	-0.77678484637023
1103	150-154	-0.714915494514127
1103	155-159	-0.4514831116690665
1103	160-164	-1.5667906533703189
1103	165-169	-0.9982411322946341
1103	170-174	-0.6390170071000405
1103	175-179	-1.0574857645014788
1103	180-184	-1.090539012227758
1103	185-189	-1.4504857018224833
1103	190-194	-2.3335035240302666
1103	195-199	-1.305244426564279
1103	200-204	-0.4454860108146228
1103	205-209	-0.8112342633387399
1103	210-214	-0.6732062389073086
1103	215-219	-0.33809542395854564
1103	220-224	-0.20102992741527004
1103	225-229	-0.7320742437739405
1103	230-234	-1.630655016628399
1103	235-239	-1.2828936465899723
1103	240-244	-0.5799329567771192
1103	245-249	-0.8303340084099702
1103	250-254	0.13360369621225487
1103	255-259	-0.06932258901832355
1103	260-264	-0.6725943163386603
1103	265-269	-0.419392911982726
1103	270-274	0.0612201089267046
1103	275-279	-0.34812021961350226
1103	280-284	-0.29533174613021806
1103	285-289	-0.3218160946814983
1103	290-294	0.28482742515001114
1103	295-299	0.28354954678319544
1103	300-301	0.24885957749163268
1104	1	0.3927847597434777
1104	2	0.4306522205122505
1104	3	0.35066816720492255
1104	4	0.48425191089143027
1104	5	0.5011649381684222
1104	6	0.43559773904497945
1104	7	0.7706820726413497
1104	8	0.01926718107738168
1104	9	0.06424313511730873
1104	10-14	0.3550196780485635
1104	15-19	0.38634649506951035
1104	20-24	0.4442027002624016
1104	25-29	0.4677000399931899
1104	30-34	0.5037061102603388
1104	35-39	0.6668432714158357
1104	40-44	0.6335623168276641
1104	45-49	0.751157035475785
1104	50-54	0.7971973132493915
1104	55-59	1.4069948439873912
1104	60-64	0.9542571905345412
1104	65-69	0.7198920344800115
1104	70-74	0.9701749435880771
1104	75-79	0.5789930530239218
1104	80-84	0.5878807327419366
1104	85-89	0.7930884682019297
1104	90-94	0.7091845556985277
1104	95-99	0.5358722231865798
1104	100-104	0.6624561639393818
1104	105-109	0.5877886935189451
1104	110-114	0.8789285508956581
1104	115-119	0.6645461133935342
1104	120-124	0.486974471922359
1104	125-129	0.6954810714171664
1104	130-134	0.11531138419676168
1104	135-139	0.6871910420060772
1104	140-144	0.23103980086662546
1104	145-149	0.4148388158307519
1104	150-154	0.36413476351266283
1104	155-159	0.6164071697453757
1104	160-164	-1.4974437280836952
1104	165-169	-0.1576559326098561
1104	170-174	-0.28590924595913236
1104	175-179	-1.6980506270990325
1104	180-184	0.19791206436680042
1104	185-189	-0.41494409976843727
1104	190-194	0.011182140865379608
1104	195-199	0.2815313824704724
1104	200-204	-0.20034021932060497
1104	205-209	0.1141133737459441
1104	210-214	-0.9144269725677319
1104	215-219	-0.8304410527558623
1104	220-224	-2.0657222018642614
1104	225-229	-0.6635280912966337
1104	230-234	-0.8418171326290818
1104	235-239	0.4213462193234889
1104	240-244	-0.5979353157069109
1104	245-249	-0.029800145352050222
1104	250-254	-0.2934246730223311
1104	255-259	-0.12558306452890733
1104	260-264	-0.022585004773697293
1104	265-269	-0.16074867584143782
1104	270-274	-0.47773327117137754
1104	275-279	0.12198965685306895
1104	280-284	-0.20042206831036857
1104	285-289	3.1620686902213517
1104	290-294	0.49982525245151876
1104	295-299	-0.24089059929237422
1104	300-301	0.07774066429929505
1105	1	0.02050723929408349
1105	2	0.09781985626666057
1105	3	0.1831556019146916
1105	4	0.157702191762624
1105	5	0.03238106116043937
1105	6	-0.022542598000455882
1105	7	-0.050151671322382185
1105	8	-0.5040155625906948
1105	9	0.1636074792584381
1105	10-14	-0.02022423640890736
1105	15-19	-0.0623821932127413
1105	20-24	0.13269683646029762
1105	25-29	-0.06604392373994017
1105	30-34	0.1519716074516282
1105	35-39	0.2018585468667098
1105	40-44	0.29250091937029055
1105	45-49	0.36086926569161193
1105	50-54	0.23549927442018515
1105	55-59	0.5734036347629896
1105	60-64	0.3616781031195444
1105	65-69	0.6388237384319169
1105	70-74	0.4905553515904444
1105	75-79	0.2935441518708757
1105	80-84	0.08793493595469926
1105	85-89	0.4038699799788077
1105	90-94	0.09491432804502864
1105	95-99	0.42724405722618997
1105	100-104	0.1409769089871773
1105	105-109	0.39960499390329574
1105	110-114	-0.1058551290097327
1105	115-119	0.012003489958040348
1105	120-124	0.7783955816099493
1105	125-129	-0.3941020566009712
1105	130-134	0.1842923637966436
1105	135-139	0.2971348677673795
1105	140-144	0.18253778056505965
1105	145-149	0.44236419278946215
1105	150-154	0.18906824138790768
1105	155-159	-0.5222101555724485
1105	160-164	-2.2134386034163036
1105	165-169	-0.33833987860375103
1105	170-174	-0.8892057868086418
1105	175-179	-1.9956410479130646
1105	180-184	-0.033624350337056796
1105	185-189	-0.12468096780825277
1105	190-194	-0.5266566292144468
1105	195-199	-0.07313009382793823
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2119	255-259	1.1902942850458071
2119	260-264	0.6847971227071383
2119	265-269	0.050581787247924836
2119	270-274	0.1437935586091541
2119	275-279	0.34124829383721256
2119	280-284	-0.09361748992120411
2119	285-289	-0.2510049210356069
2119	290-294	-0.6748255928703326
2119	295-299	-0.05942550080795428
2119	300-301	0.4211969394026891
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	30.0
11	119.0
12	277.0
13	529.0
14	616.0
15	1187.0
16	1995.0
17	2469.0
18	2581.0
19	2398.0
20	2566.0
21	2741.0
22	3067.0
23	3499.0
24	4455.0
25	5394.0
26	6406.0
27	7470.0
28	7651.0
29	6953.0
30	4903.0
31	3307.0
32	4133.0
33	6225.0
34	9007.0
35	9108.0
36	4662.0
37	388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.32492125681802	0.1411615579626642	0.20550049934700776	0.3284166858723208
2	0.7240531612506722	0.2439118076361681	0.16228777752170237	98.86974725359146
3	98.5269263271107	1.062072674195283	0.2794422678036414	0.13155873089037415
4	0.34570177460244295	97.44372743335637	1.1456172697242069	1.0649535223169702
5	0.349542905431359	0.8652147192133364	97.35346085887684	1.431781516478451
6	1.7525159406929398	0.1411615579626642	0.8632941537988783	97.24302834754552
7	96.81666282553584	0.1296381654759161	2.6388568794653144	0.41484212952293154
8	0.4129215641084735	1.1446569870169778	96.7225551202274	1.7198663286471536
9	0.7778289928554967	2.1673580702158715	0.468617961127756	96.58619497580088
10-14	20.234885150188216	19.561919028962127	40.20742106476147	19.995774756088192
15-19	0.8752840303401066	0.8962201636091589	39.18379812189439	59.04469768415635
20-24	5.1540293462395335	69.55826995467466	4.811016363217331	20.47668433586848
25-29	0.7474840593070601	20.176499961588693	46.840669893216564	32.235346085887684
30-34	0.41676269493738954	20.774371975109474	39.0712145655681	39.73765076438504
35-39	0.49569793347161406	39.54770684489514	20.175731735422907	39.780863486210336
40-44	30.031497272797115	68.33506184220634	0.9501037105323807	0.6833371744641622
45-49	39.47261273718983	56.47537835138665	3.106322501344396	0.9456864100791273
50-54	56.94610893447031	29.526964738418993	5.698509641238381	7.8284166858723205
55-59	25.51048634028837	26.698771759300165	41.97955365341099	5.811188247000473
60-64	39.76300222785588	1.6080894215256971	24.87381885227011	33.75508949834831
65-69	28.43933611329329	47.98984309424525	23.12098203910255	0.44983875335890483
70-74	11.788622570484751	29.432472920027656	38.87205193208881	19.906852577398787
75-79	69.700583851886	22.647115310747484	0.8755857724514097	6.776715064915111
80-84	28.93916550631392	28.54621404907092	24.798962884716953	17.715657559898208
85-89	34.492533730253996	2.122821337686657	33.04402938493302	30.340615547126326
90-94	1.2116963556921303	21.45849138137994	36.00556969318673	41.3242425697412
95-99	35.579584193594854	57.68492821817832	0.6695155327219475	6.065972055504874
100-104	41.96610169491525	20.156911701157153	14.129159264416383	23.74782733951121
105-109	21.16464205118356	42.200989100686606	5.780573294281462	30.853795553848368
110-114	42.79965656398801	30.83778794939121	23.424525710641714	2.9380297759790714
115-119	50.376445280989515	25.275995851419353	3.6941574155879078	20.653401452003227
120-124	40.060429576301466	22.715835007664147	18.628098792560976	18.595636623473414
125-129	45.573683573556785	32.62505354841064	2.2141667483099834	19.587096129722585
130-134	45.137567102976064	33.1194360949189	20.81397470493897	0.9290220971660696
135-139	1.36693203752965	21.005080139439745	38.564500484966054	39.06348733806455
140-144	50.40842784569437	1.9926822944176086	38.481336009449635	9.117553850438389
145-149	51.63501765250496	27.92822211038674	18.613500420663613	1.8232598164446816
150-154	40.17074646358913	39.19101900490728	1.0277438995111927	19.610490631992395
155-159	4.854654233609588	32.904898637293414	8.105559343519221	54.13488778557778
160-164	38.37742843149494	40.32631973187619	4.061998828398844	17.23425300823002
165-169	65.0184863297193	24.760734075347397	9.168643343480808	1.0521362514524975
170-174	6.957774341934679	10.702288463569927	29.54393984500293	52.79599734949246
175-179	21.504066992538245	16.63462369515322	35.29573325906791	26.56557605324063
180-184	21.785059204271544	37.175672950418225	36.835908615109815	4.203359230200421
185-189	7.200929598294456	21.188887074934456	65.69916739491602	5.911015931855068
190-194	38.31280724301085	46.752613066388506	13.836909310603835	1.0976703799968113
195-199	2.6710585704545236	22.31266385610433	18.20687403366913	56.80940353977202
200-204	2.295578821211056	39.489064693759566	18.54229208321089	39.673064401818486
205-209	2.3704984154422357	33.07519446845289	10.894650917122828	53.65965619898204
210-214	3.845577643330452	37.28858158071641	19.12033035628541	39.745510419667724
215-219	58.20144437615242	35.69280116779348	3.636677934849416	2.4690765212046712
220-224	40.95690487083764	2.970022952798742	35.507825866304756	20.565246310058864
225-229	40.60752813289149	19.060935525624924	3.196311903420183	37.1352244380634
230-234	8.039566342130758	12.06113575660017	59.532970901044216	20.366327000224857
235-239	40.15668037697163	21.00517533133237	20.543842096812046	18.294302194883958
240-244	38.92472786227129	52.01019068941558	2.9061993360611442	6.1588821122519875
245-249	47.19235770298693	6.917724236125482	13.803327598933834	32.086590461953755
250-254	43.74183586813866	18.83764378908361	29.043464834401295	8.377055508376438
255-259	58.61540789510254	35.15738633380788	4.440416155839183	1.7867896152504008
260-264	53.87993329627571	25.769254524118335	14.994132542770675	5.3566796368352785
265-269	53.99635600024705	5.510468778951269	28.055709962324748	12.437465258476932
270-274	39.369402754616765	28.618059415724783	15.139892532888641	16.87264529676981
275-279	47.489345932925694	13.346921129022297	33.65233771848558	5.511395219566426
280-284	35.921808412080786	20.955160274226422	29.30547835217096	13.817552961521834
285-289	62.159215841290624	8.14237891122009	17.96814200832582	11.730263239163465
290-294	54.917361881140714	7.2433060189460745	20.369964263762466	17.469367836150738
295-299	51.02968011022977	10.26794026738167	25.138718206531856	13.563661415856702
300-301	32.327610576107645	8.200629440476416	36.53110453611745	22.940655447298493
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.25
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.25
33	0.25
34	0.25
35	0.0
36	0.3333333333333333
37	0.9166666666666665
38	1.3333333333333333
39	2.5
40	4.583333333333333
41	9.666666666666666
42	12.833333333333334
43	20.99999999999999
44	40.66666666666668
45	253.666666666669
46	1631.3333333332453
47	2986.4166666668984
48	2574.416666666719
49	4127.250000000517
50	6383.416666665669
51	8187.3333333314895
52	5589.916666666365
53	3914.0000000001023
54	2674.583333333348
55	1581.1666666666572
56	928.250000000004
57	643.916666666667
58	440.24999999999966
59	278.6666666666667
60	135.0833333333333
61	57.500000000000014
62	23.91666666666667
63	7.333333333333334
64	3.1666666666666665
65	5.166666666666666
66	3.9166666666666665
67	0.8333333333333333
68	1.0833333333333333
69	0.5833333333333333
70	0.3333333333333333
71	0.3333333333333333
72	0.25
73	0.5
74	0.25
75	0.0
76	0.25
77	0.25
78	0.0
79	0.25
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.25
90	0.0
91	0.25
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.00941077053084428
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.01882154106168856
60-64	0.0
65-69	0.00941077053084428
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.009410951274319627
115-119	0.0
120-124	0.009411222402550634
125-129	0.018822444805101268
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.009411222402550634
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.018822444805101268
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	7.682792848856416E-4
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.014822820968115496
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0024705083070841825
290-294	0.016058651135068696
295-299	0.0
300-301	0.04050146464598894
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	1.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	3.0
115-119	1.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	1.0
205-209	0.0
210-214	0.0
215-219	15.0
220-224	104.0
225-229	116.0
230-234	39115.0
235-239	15.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	1.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	2.0
295-299	7627.0
300-302	57135.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.375508949834831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.09775451629241	8.770261965122533
2	8.509201418200236	1.9359299377736807
3	4.0942090157015025	1.397211339018207
4	2.557825426304238	1.163862641161558
5	1.764308627384771	1.0034954290543134
6	1.0383251730541954	0.708688637935008
7	0.8272834712139119	0.6587539371590997
8	0.6415667735944622	0.583851885995237
9	0.40520006753334453	0.41484212952293154
>10	2.6253587708931283	5.083736652070369
>50	0.16039169339861556	1.1581009449181838
>100	0.07597501266250212	1.5441345932242452
>500	0.008441668073611346	0.8671352846277944
>1k	0.16039169339861556	43.58723208112468
>5k	0.025325004220834033	18.751440424060846
>10k+	0.008441668073611346	12.371322117231314
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCGATCGTCACTGTTCTCCAACAATCTCATCATCACCATAGAAGGAGAAA	12883	12.371322117231314	No Hit
GCGATCGTCACTGTTCTCCAACAATTTCATCATCACCATAGAAGGAGAAA	7184	6.898670968733195	No Hit
GCGATCGTCACTGTTCTCCAACAATCTCATCATCACCATAAAAGGAGAAC	7010	6.731581777675348	No Hit
GCGATCGTCACTGTTCTCCAACGATCTCATCATCACCATAGAAGGAGAAA	5333	5.121187677652301	No Hit
GCGATCGTCACTGTTCTCCAACTATCTCATCATCACCATAGAAGGAGAAA	4421	4.245409848659445	No Hit
GCGATCGTCACTGTTCTCCAACAATTTCATCATCACCATAAAAGGAGAAC	4107	3.943881078589537	No Hit
GCGATCGTCACTGTTCTCCAACAATCTCATCATCACCATAGAAAGAGAAG	3970	3.8123223476991623	No Hit
GCGATCGTCACTGTTCTCCAACAATCTCATCATCACCATAGAAAGAGTAC	3681	3.5348006453099794	No Hit
GCGATCGTCACTGTTCTCCAACGATTTCATCATCACCATAGAAGGAGAAA	3478	3.339863255742491	No Hit
GCGATCGTCACTGTTCTCCAACTATTTCATCATCACCATAGAAGGAGAAA	3238	3.1093954060075286	No Hit
GCGATCGTCACTGTTCTCCAACGATCTCATCATCACCATAAAAGGAGAAC	2863	2.7492893907966507	No Hit
GCGATCGTCACTGTTCTCCAACAATTTCATCATCACCATAGAAAGAGAAG	2360	2.2662671890604593	No Hit
GCGATCGTCACTGTTCTCCAACTATCTCATCATCACCATAAAAGGAGAAC	2317	2.224975032649612	No Hit
GCGATCGTCACTGTTCTCCAACAATTTCATCATCACCATAGAAAGAGTAC	2067	1.98490435584236	No Hit
GCGATCGTCACTGTTCTCCAACGATTTCATCATCACCATAAAAGGAGAAC	2043	1.961857570868864	No Hit
GCGATCGTCACTGTTCTCCAACTATTTCATCATCACCATAAAAGGAGAAC	1733	1.664169931627871	No Hit
GCGATCGTCACTGTTCTCCAACGATCTCATCATCACCATAGAAAGAGAAG	1611	1.5470154413459323	No Hit
GCGATCGTCACTGTTCTCCAACGATCTCATCATCACCATAGAAAGAGTAC	1499	1.4394637781362833	No Hit
GCGATCGTCACTGTTCTCCAACTATCTCATCATCACCATAGAAAGAGTAC	1363	1.3088653299531383	No Hit
GCGATCGTCACTGTTCTCCAACTATCTCATCATCACCATAGAAAGAGAAG	1345	1.291580241223016	No Hit
GCGATCGTCACTGTTCTCCAACGATTTCATCATCACCATAGAAAGAGAAG	1192	1.1446569870169778	No Hit
GCGATCGTCACTGTTCTCCAACGATTTCATCATCACCATAGAAAGAGTAC	1073	1.030383344856726	No Hit
GCGATCGTCACTGTTCTCCAACTATTTCATCATCACCATAGAAAGAGAAG	1029	0.9881309057386495	No Hit
GCGATCGTCACTGTTCTCCAACTATTTCATCATCACCATAGAAAGAGTAC	903	0.8671352846277944	No Hit
GCGATCGTCACTGTTCTCCAACAATCTCATCATCACCATAGAAGGAGAAC	380	0.36490742874702314	No Hit
GCGATCGTCACTGTTCTCCAACAATTTCATCATCACCATAGAAGGAGAAC	232	0.22278558807712992	No Hit
GCGATCGTCACTGTTCTCCAACAATCTCATCATCACCATAAAAGGAGAAA	196	0.1882154106168856	No Hit
GCGATCGTCACTGTTCTCCAACGATCTCATCATCACCATAGAAGGAGAAC	171	0.1642083429361604	No Hit
GCGATCGTCACTGTTCTCCAACAATCTCATCATCACCATAGAAAGAGAAA	151	0.14500268879158024	No Hit
GCGATCGTCACTGTTCTCCAACTATCTCATCATCACCATAGAAGGAGAAC	144	0.13828070984097718	No Hit
GCGATCGTCACTGTTCTCCAACGATTTCATCATCACCATAGAAGGAGAAC	114	0.10947222862410692	No Hit
GCGATCGTCACTGTTCTCCAACAATTTCATCATCACCATAAAAGGAGAAA	114	0.10947222862410692	No Hit
GCGATCGTCACTGTTCTCCAACAATCTCATCATCACCATAGAAGGAGAAG	106	0.10178996696627488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	1.9205654144580165E-4	0.0	0.0	0.0	0.0
105-109	9.602827072290081E-4	0.0	0.0	0.0	0.0
110-114	9.602827072290081E-4	0.0	0.0	0.0	0.0
115-119	9.602827072290081E-4	0.0	0.0	0.0	0.0
120-124	9.602827072290081E-4	0.0	0.0	0.0	0.0
125-129	9.602827072290081E-4	0.0	0.0	0.0	0.0
130-134	9.602827072290081E-4	0.0	0.0	0.0	0.0
135-139	9.602827072290081E-4	0.0	0.0	0.0	0.0
140-144	9.602827072290081E-4	0.0	0.0	0.0	0.0
145-149	9.602827072290081E-4	0.0	0.0	0.0	0.0
150-154	9.602827072290081E-4	0.0	0.0	0.0	0.0
155-159	9.602827072290081E-4	0.0	0.0	0.0	0.0
160-164	9.602827072290081E-4	0.0	0.0	0.0	0.0
165-169	9.602827072290081E-4	0.0	0.0	0.0	0.0
170-174	9.602827072290081E-4	0.0	0.0	0.0	0.0
175-179	9.602827072290081E-4	0.0	0.0	0.0	0.0
180-184	9.602827072290081E-4	0.0	0.0	0.0	0.0
185-189	9.602827072290081E-4	0.0	0.0	0.0	0.0
190-194	9.602827072290081E-4	0.0	0.0	0.0	0.0
195-199	9.602827072290081E-4	0.0	0.0	0.0	0.0
200-204	0.0017285088730122147	0.0	0.0	0.0	0.0
205-209	0.0019205654144580162	0.0	0.0	0.0	0.0
210-214	0.0019205654144580162	0.0	0.0	0.0	0.0
215-219	0.002112621955903818	0.0	0.0	0.0	0.0
220-224	0.014020127525543522	0.0	0.0	1.9205654144580165E-4	0.0
225-229	0.022854728432050397	0.0	0.0	9.602827072290081E-4	0.0
230-234	2.2683798110163633	0.0	0.0	9.602827072290081E-4	0.0
235-239	10.13079050472459	0.0	0.0	9.602827072290081E-4	0.0
240-244	10.132903126680494	0.0	0.0	9.602827072290081E-4	0.0
245-249	10.132903126680494	0.0	0.0	9.602827072290081E-4	0.0
250-254	10.132903126680494	0.0	0.0	9.602827072290081E-4	0.0
255-259	10.132903126680494	0.0	0.0	9.602827072290081E-4	0.0
260-264	10.132903126680494	0.0	0.0	9.602827072290081E-4	0.0
265-269	10.132903126680494	0.0	0.0	9.602827072290081E-4	0.0
270-274	10.134823692094953	0.0	0.0	9.602827072290081E-4	0.0
275-279	10.134823692094953	0.0	0.0	9.602827072290081E-4	0.0
280-284	10.134823692094953	0.0	0.0	9.602827072290081E-4	0.0
285-289	10.134823692094953	0.0	0.0	9.602827072290081E-4	0.0
290	10.134823692094953	0.0	0.0	9.602827072290082E-4	0.0
>>END_MODULE