Assembly

Learn all about Genome assembly. Start with the tutorial at your own pace. If you need support contact us via the Slack Channel gta_assembly.

Organisers(s) orcid logoDelphine Lariviere avatar Delphine Lariviere
Instructor(s) orcid logoAnna Syme avatar Anna Symeorcid logoBjörn Grüning avatar Björn Grüningorcid logoClea Siguret avatar Clea Siguretorcid logoDelphine Lariviere avatar Delphine LariviereGareth Price avatar Gareth PriceIgor Makunin avatar Igor MakuninMichael Schatz avatar Michael Schatzorcid logoSaim Momin avatar Saim MominNguyen Vo Minh Trung avatar Nguyen Vo Minh Trung

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Introduction

In this section, we will introduce what is a genome assembly, how it works, and the metrics to evaluate the quality of an assembly.

Lesson Slides Hands-on Recordings
An introduction to get started in genome assembly and annotation

Introduction To Genome Assembly

Lecture Slides; Lecture Video

ERGA post-assembly QC

Genome Assembly

In this section, we will present genome assemblies for different types of organisms.

Lesson Slides Hands-on Recordings
Genome Assembly of a bacterial genome (MRSA) sequenced using Illumina MiSeq Data
Using the VGP workflows to assemble a vertebrate genome with HiFi and Hi-C data
Assembly of metagenomic sequencing data

Non-nuclear genome assembly

When sequencing organisms with non-nuclear DNA, the data contain sequencing for both nuclear and non-nuclear DNA. In this section we will learn how to assemble organelles genome.

Lesson Slides Hands-on Recordings

Mitochondrial Genome Assembly

Tutorial (will come soon)

Chloroplast genome assembly

Assembly decontamination

In this section we will learn how remove sequences that do not belong to the organism you want to sequence.

Lesson Slides Hands-on Recordings

Assembly decontamination

Tutorial (will come soon)