Genome annotation for eukaryotes
purlPURL: https://gxy.io/GTN:P00002Comment: What is a Learning Pathway?We recommend you follow the tutorials in the order presented on this page. They have been selected to fit together and build up your knowledge step by step. If a lesson has both slides and a tutorial, we recommend you start with the slides, then proceed with the tutorial.
Learn how to annotate an eukaryotic genome sequence: identify repeated regions, find the position and function of genes, and even set up a manual curation environment with Apollo.
Module 1: Introduction
General introduction to genome annotation
Lesson | Slides | Hands-on | Recordings |
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Introduction to Genome Annotation
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Module 2: Repeat Masking
Learn how to identifying, and “mask”, repeated regions, a first step before annotating genes
Lesson | Slides | Hands-on | Recordings |
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Masking repeats with RepeatMasker |
Module 3: Gene calling and functional annotation
In this module you will learn to run the Funannotate tool suite to find the position of genes and to functionally annotate them. Optionally you can also identify long non-coding RNAs.
Lesson | Slides | Hands-on | Recordings |
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Genome annotation with Funannotate | |||
Long non-coding RNAs (lncRNAs) annotation with FEELnc |
Module 4: Manual curation
Automatic annotation is rarely perfect, in this module you will learn how to start a collaborative manual curation project using Galaxy and Apollo
Lesson | Slides | Hands-on | Recordings |
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Refining Genome Annotations with Apollo (eukaryotes) |
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Anthony BretaudeauFunding
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