Identification of the micro-organisms in a beer using Nanopore sequencing

microbiome-beer-data-analysis/main-workflow

Author(s)
Bérénice Batut, Teresa Müller, Polina Polunina
Version
1
Last updated
Jan 15, 2024
License
MIT
Tags
Microbiome

Features
Tutorial
Identification of the micro-organisms in a beer using Nanopore sequencing
Workflow Testing
Tests: ✅
Results: Not yet automated
FAIRness PURL
https://gxy.io/GTN:W00121
RO-Crate logo with flask Download Workflow RO-Crate Workflowhub cloud with gears logo View on (Dev) WorkflowHub
Launch in Tutorial Mode
Download 
flowchart TD
  0["ℹ️ Input Dataset\ninput"];
  style 0 stroke:#2c3143,stroke-width:4px;
  1["FastQC"];
  0 -->|output| 1;
  2["Porechop"];
  0 -->|output| 2;
  3["fastp"];
  2 -->|outfile| 3;
  4["Kraken2"];
  3 -->|out1| 4;
  7500e09c-a7a5-4b18-91b5-fe769bc16111["Output\nkraken_report"];
  4 --> 7500e09c-a7a5-4b18-91b5-fe769bc16111;
  style 7500e09c-a7a5-4b18-91b5-fe769bc16111 stroke:#2c3143,stroke-width:4px;
  5["Filter"];
  4 -->|report_output| 5;
  6["Filter"];
  4 -->|report_output| 6;
  7["Krakentools: Convert kraken report file"];
  4 -->|report_output| 7;
  0803bec5-7aad-446c-8d5c-03a62b0ab74b["Output\nkrakentool_report"];
  7 --> 0803bec5-7aad-446c-8d5c-03a62b0ab74b;
  style 0803bec5-7aad-446c-8d5c-03a62b0ab74b stroke:#2c3143,stroke-width:4px;
  8["Krona pie chart"];
  7 -->|output| 8;

Inputs

Input Label
input input

Outputs

From Output Label
toolshed.g2.bx.psu.edu/repos/iuc/kraken2/kraken2/2.1.1+galaxy1 Kraken2
toolshed.g2.bx.psu.edu/repos/iuc/krakentools_kreport2krona/krakentools_kreport2krona/1.2+galaxy0 Krakentools: Convert kraken report file

Tools

Tool Links
Filter1
toolshed.g2.bx.psu.edu/repos/crs4/taxonomy_krona_chart/taxonomy_krona_chart/2.7.1+galaxy0 View in ToolShed
toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.73+galaxy0 View in ToolShed
toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.2+galaxy0 View in ToolShed
toolshed.g2.bx.psu.edu/repos/iuc/kraken2/kraken2/2.1.1+galaxy1 View in ToolShed
toolshed.g2.bx.psu.edu/repos/iuc/krakentools_kreport2krona/krakentools_kreport2krona/1.2+galaxy0 View in ToolShed
toolshed.g2.bx.psu.edu/repos/iuc/porechop/porechop/0.2.4+galaxy0 View in ToolShed

To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.

Importing into Galaxy

Below are the instructions for importing these workflows directly into your Galaxy server of choice to start using them!
Hands-on: Importing a workflow
  • Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
  • Click on Import at the top-right of the screen
  • Provide your workflow
    • Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
    • Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
  • Click the Import workflow button

Below is a short video demonstrating how to import a workflow from GitHub using this procedure:

Video: Importing a workflow from URL

Version History

Version Commit Time Comments
1 0e0a2f2cc 2024-01-10 15:47:09 Rename metagenomics topic to microbiome

For Admins

Installing the workflow tools

wget https://training.galaxyproject.org/training-material/topics/microbiome/tutorials/beer-data-analysis/workflows/main_workflow.ga -O workflow.ga
workflow-to-tools -w workflow.ga -o tools.yaml
shed-tools install -g GALAXY -a API_KEY -t tools.yaml
workflow-install -g GALAXY -a API_KEY -w workflow.ga --publish-workflows