Workflows

These workflows are associated with Identifying Mycorrhizal Fungi from ITS2 sequencing using LotuS2

To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.

Workflow for Identifying MF from ITS2 sequencing using LotuS2 - tutorial example run'
Society for the Protection of Underground Networks, Sujai Kumar, Bethan Manley

Last updated Sep 30, 2024

Launch in Tutorial Mode question
License: MIT
Tests: ✅ Results: Not yet automated

flowchart TD
  0["ℹ️ Input Dataset\nMapping tsv"];
  style 0 stroke:#2c3143,stroke-width:4px;
  1["ℹ️ Input Dataset\nsdm Options txt"];
  style 1 stroke:#2c3143,stroke-width:4px;
  2["ℹ️ Input Collection\nInput Dataset Collection"];
  style 2 stroke:#2c3143,stroke-width:4px;
  3["LotuS2"];
  2 -->|output| 3;
  0 -->|output| 3;
  1 -->|output| 3;
  7ff13687-fb95-4b54-8b5c-4c916500dfed["Output\nOTU Table"];
  3 --> 7ff13687-fb95-4b54-8b5c-4c916500dfed;
  style 7ff13687-fb95-4b54-8b5c-4c916500dfed stroke:#2c3143,stroke-width:4px;
  b3413904-3319-4fa0-8c17-2c4757af5b8c["Output\nOTU fasta"];
  3 --> b3413904-3319-4fa0-8c17-2c4757af5b8c;
  style b3413904-3319-4fa0-8c17-2c4757af5b8c stroke:#2c3143,stroke-width:4px;
  11ad8056-9572-428a-a3b2-5ceec0fa186e["Output\nComplete Output Zip"];
  3 --> 11ad8056-9572-428a-a3b2-5ceec0fa186e;
  style 11ad8056-9572-428a-a3b2-5ceec0fa186e stroke:#2c3143,stroke-width:4px;
  21d55970-8b95-4dca-9b46-e7c65340ce84["Output\nOTU Phylogenetic Tree"];
  3 --> 21d55970-8b95-4dca-9b46-e7c65340ce84;
  style 21d55970-8b95-4dca-9b46-e7c65340ce84 stroke:#2c3143,stroke-width:4px;
	

Importing into Galaxy

Below are the instructions for importing these workflows directly into your Galaxy server of choice to start using them!
Hands-on: Importing a workflow
  • Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
  • Click on galaxy-upload Import at the top-right of the screen
  • Provide your workflow
    • Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
    • Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
  • Click the Import workflow button

Below is a short video demonstrating how to import a workflow from GitHub using this procedure:

Video: Importing a workflow from URL